CHONGQING CAMAB BIOTECH LTD

1.CAMouse^HGplatform

Expresses human mouse chimeric IgM (Human Fv, mouse IgCμ and human light chain) and fully human IgG.

Human IgH heavy chain (hIgH) has 24 human variable-regions and all human D-regions and J-regions; mouse IgH 5’-enhance elements and mouse IgM (including Sμ, IgM (CH1, CH2, CH3 and CH4), TM1, TM2 and PolyA) are engineered for B-cell development and BCR signalling; human Igγ3 and Igγ1, and 3’-Locual control region are constructed for class switch and IgG production. In particular, the replacement of human IgH 5’-enhancer and IgM with mouse IgH 5’-enhance elements and mouse IgM in the transgene promotes normal B-cell development, class-switch and hyper-mutation.

Human light chains (hIgK and hIgL) are composed of fully human immunoglobulin genomic segments.

Mouse endogenous IgH and IgK are inactivated.

2.CAMouse^MG platform

Produces human and mouse chimeric IgM and IgG. Fc grafting in vitro to yield fully human antibody.

Transgenic IgH heavy chain (IgH) has 24 human variable-regions, all human D-regions and J-regions plus mouse C-region and mouse 3’-local control region.

Human light chains (hIgK and hIgL) transgenes are the same as CAMouse^HG.

Mouse endogenous IgH and IgK are inactivated.

3. CAMouse^H platform

Generates fully human heavy chain-only antibody (Igγ1-dCH1, Nanobody)

Human heavy chain-only IgH constitutes with 24 human variable-regions and all the human D-regions and J-regions, mouse IgH 5’-enhance elements and human Igγ1 [including mouse Sγ, human Igγ1 (Hinge, CH2 and CH3), mouse TM1, TM2 and PolyA] and mouse 3-Locual control region. In particular, mouse enhancer and regulatory elements control human Igγ1-dCH1 expression, which promotes normal B-cell development and hyper-mutation.

Mouse endogenous IgH and IgK are inactivated.